Pricing For Core Service Support for Duke and Non-Duke Scientists
Note: Scientific collaborations with academic, government, and non-profit laboratories outside Duke are welcome. Contact us for details.
Please note: Prices below are based on an a-la-carte menu of services to maximize flexibility in experimental designs and comply with auditing regulations. Most analyses will require combinations of these services. Please see the FAQs for additional information. All prices are effective August 1, 2021.
Sample Preparation (Proteomics)
| Duke | Non-Duke | |
| Mini-Bradford Assay (per plate, 20 samples max) | $44 | $71 |
| - NOTE - Bradford Assays required for all solution phase analyses | ||
| General Sample Preparation - Proteomics (cost per method) | $57 | $92 |
| In-Solution Digestion* | ||
| FFPE Protein Extraction | ||
| In-situ/bead-bound digestion | ||
| Tissue Disruption/Lysis and Protein Extraction (RapiGest, Urea,TriZol, or Bead Blasting) | $28 | $45 |
| In-Gel Digestion (per spot or band)* | $60 | $97 |
| S-trap Digestion | $101 | $163 |
| Sample Cleanup Procedures (cost per method) | $47 | $76 |
| Solid Phase Extraction (C18, HLB, MCX) | ||
| Buffer exchange by ZebaSpin | ||
| Protein Precipitation and Resuspension | ||
| Biotin-Avidin enrichment | ||
| Amicon MW Cutoff Filtration | $62 | $100 |
| TiO2 enrichment for phosphopeptides | $59 | $95 |
| Glycopeptide enrichment | $107 | $172 |
| 1D PAGE Gel Separation (Protein Level) | $124 | $200 |
| OFFGEL pl Fractionation (12 or 24 fraction, peptide or protein) | Contact | Contact |
| GELFREE Molecular Weight Fractionation (protein level) | Contact | Contact |
| Offline Fractionation (high pH C18) | $189 | $304 |
| Biofluid Immunodepletion, including MW filter cleanup | ||
| Spin Column Format (Pierce Hu-12 or Agilent MU-3) | $136 | $219 |
| LC Format (Agilent MARS-14) | $210 | $338 |
| Antibody-Based Enrichment (not including antibody reagent costs) | $76 | $122 |
| *Additional reagents charge for digestions with enzymes other than Trypsin* | Contact | Contact |
Sample Preparation (Metabolomics)
| Duke | Non-Duke | |
| MeOH Precipitation with Internal Standards - Biofluids | included | included |
| Solid Sample Solubilization (Cells, Tumor) by Bead Blasting, Probe Sonication, or Tissue Tearing | $28 | $45 |
| Mini-Bradford Assay (per plate, 20 samples max) | $44 | $71 |
| -NOTE - this is used to normalize to total biomass | ||
| Specialized Per-Sample Preparation for Specific Assays is Included in the Cost/Sample | n/a | n/a |
| Pharmacokinetics Plate Prep (biofluids) | $76 | $122 |
Qualitative Analyses (Proteomics)
| Duke | Non-Duke | |
| LC-MS/MS analysis (simple in-gel digest - very fast gradient) | $93 | $150 |
| LC-MS/MS analysis (in-gel or solution digest of simple sample - fast gradient) | $160 | $258 |
| LC-MS/MS analysis (moderately complex - medium gradient) | $203 | $327 |
| LC-MS/MS analysis (complex sample - slow gradient) | $240 | $386 |
| LC-MS/MS analysis (very complex sample - very slow gradient) | $285 | $459 |
| Molecular Weight Determination (per sample) | $68 | $109 |
| MW Setup Fee (per order) | $156 | $251 |
| ZipChip Profiling Method | $70 | $113 |
Quantitative Analyses, Unbiased (Proteomics)
| Duke | Non-Duke | |
| LC-MS/MS Differential Expression (per sample), 15-min. gradient | $104 | $167 |
| LC-MS/MS Differential Expression (per sample), 30-min. gradient | $260 | $419 |
| LC-MS/MS Differential Expression (per sample), 60-min. gradient | $304 | $489 |
| *LC-MS/MS Differential Expression (per sample), 90-min. gradient | $349 | $562 |
| LC-MS/MS Differential Expression (per sample), 120-min. gradient | $388 | $625 |
| Quantitative 1D Workup - Proteome Discoverer, Spectronaut or Elucidator (analysis per sample) | $149 | $240 |
Quantitative Analyses, Targeted (Proteomics)
*Stable Isotope Labeled Peptides are provided at third party vendor cost. Methods are developed and performed on nanoAcquity UPLC and TQ-S triple quadrupole mass spectrometer unless otherwise stated. The shortest method possible is used for quantification of the number of analytes requested, using retention-time scheduled MRM technology. Typically, at least two peptides per protein and three transitions per peptide are used for MRM quantitative assays.
| Duke | Non-Duke | |
| Targeted (MRM) Quantitation Method Development, priced per hour | Contact | Contact |
| MRM Quantitative Data Collection (30 min gradient) | $125 | $201 |
| MRM Quantitative Data Collection (60 min gradient) | $169 | $272 |
| MRM Quantitative Data Collection (90 min gradient) | $205 | $330 |
| MRM Data Analysis in Skyline, priced per hour | Contact | Contact |
Quantitative Analysis (Metabolomics)
All prices are on a per-sample basis.
| Duke | Non-Duke | |
| AbsoluteIDQ p180 Panel (kit from Biocrates, Inc.)* | $56 | $90 |
| Absolute IDQ p400 HR Panel (kit from Biocrates, Inc.)* | $77 | $124 |
| MxP Quant 500 Panel (kit from Biocrates, Inc.)* | $84 | $135 |
| Bile Acids Panel (kit from Biocrates, Inc.)* | $41 | $66 |
| Oxylipins/Eicosanoid Panel** | $156 | $251 |
| Energy Metabolite Panel | $68 | $109 |
| Methionine Pathway Panel** | $94 | $151 |
| Hydroxycholesterols Panel** | $139 | $224 |
| 27-Hydroxycholesterol quantitation** | $82 | $132 |
| Fatty Acids Panel | $80 | $129 |
| Fast HR Metabolite Profiling (RPLC OR HILIC, ESI+ OR ESI-) | $153 | $246 |
| High Resolution Polar Metabolite Profiling, ESI+ | $222 | $357 |
| High Resolution Lipid Profiling, ESI+ and ESI- | $271 | $436 |
| PK Analysis - method development | Contact | Contact |
| per sample with in-house developed method** | $32 | $52 |
| Polyamine Panel** | $61 | $98 |
| Short Chain Fatty Acids Analysis** | $65 | $105 |
| Sphingosine S1P Panel** | $47 | $76 |
| ZipChip Profiling Method | $70 | $113 |
*These assays require additional purchase of kit consumables from Biocrates, Inc. Negotiated prices may vary significantly depending on the number of samples to be analyzed.
**These assays will incur an extra charge for running a standard curve, quality control injections, and blanks.
Data Analysis
| Duke | Non-Duke | |
| Custom Database Setup for MASCOT or Identify | $27 | $43 |
| Additional Database Searches | $35 | $56 |
| Consulting and Extensive Data Mining (per hour) | $70 | $113 |
FAQ Total Pricing Examples
Total Pricing for Common Workflows:
| Duke | Non-Duke | |
| Digestion and Very Fast LC-MS/MS of 1D Gel Band for simple ID | $153 | $247 |
| (In-gel Digestion, 20-min gradient LC-MS/MS) | ||
| Analysis of Immunoprecipitation Samples (2) by 90-min gradient | $638 | $1,027 |
| (Bradford assay, in-solution digestion, 90-min LC-MS/MS) | ||
| Quantitative 1DLC Analysis between two conditions, requiring n=3 biol samples per condition. 1DLC, with coverage of ~1,000 proteins | $3,374 | $5,435 |
| (Bradford assay, in-solution digestion, quantitative LC-MS/MS, and quantitative workup including statistics and M&M for publication) | ||
| Qualitative Phosphorylation Mapping from a Simple Sample, i.e. gel band | $322 | $519 |
| (In-gel digestion, TiO2 enrichment, Medium 1D LC-MS/MS) | ||
| Qualitative Phosphorylation Mapping from a Complex Sample | $400 | $644 |
| (Bradford, macroscale digestion, TiO2 enrichment, Slow 1D LC-MS/MS) |
For additional pricing examples for your particular study, contact us.