Event sponsored by:
Computational Biology and Bioinformatics (CBB)
Biostatistics and Bioinformatics
Center for Advanced Genomic Technologies
Developmental and Stem Cell Biology Program
Duke Center for Genomic and Computational Biology (GCB)
Molecular Genetics and Microbiology (MGM)
School of Medicine (SOM)
University Program in Genetics & Genomics (UPGG)
Contact:
Franklin, MonicaSpeaker:
John Doench
The CRISPR toolbox allows functional interrogation of the genome in the service of three foundational questions: 1) What genes are involved in my phenotype of interest? 2) How does function arise from a genetic locus? 3) How do genes interact to produce a phenotype? The Genetic Perturbation Platform (GPP) at the Broad Institute engages in dozens of collaborative screening projects each year, and is also the home for >1,700 genetic screens reported in the Cancer Dependency Map (DepMap). We thus aim to continually incorporate new technologies and best practices in service of these questions, whether derived in-house or learned from the literature, quickly and at scale. Here, we will describe advances to our screening strategies across numerous CRISPR technologies, including knockout, activation, interference, and base editing.
CBB Monday Seminar Series