Solutions to make:
2% formic acid in water
5% ammonium hydroxide in methanol
- Dilute samples to ~1 mL with 2% formic acid in water.
- Draw through 2 x 1 mL of methanol.
- Draw through 2 x 1 mL of 2% formic acid in water.
- Draw through diluted sample.
- Draw through 2 x 1 mL of 2% formic acid in water.
- Draw through 2 x 1 mL of methanol.
- Draw through 2 x 750 uL of 5% ammonium hydroxide in methanol, collecting eluate in a suitable device.
- Speed Vac to dryness